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Influenza virus A H5N1. You are highly recommended to post your data including images for the troubleshooting.
This action might not be possible to undo. Your questions will be directed to the authors of the protocol. For measurement of ms 2 modification in multiple samples, it is recommended to prepare a master mixture of all reagents except RNA to reduce variability. De sc ri pt io n In st ru me nt rx ns Pr ic e Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs governs protein translation and contributes to myopathy in mice and humans.
LightMix Influenza a en | Influenza A Virus | Reverse Transcription Polymerase Chain Reaction
Online Technical Support Find help. Not for use in diagnostic procedures unless otherwise indicated. The representative amplification curves using r1 primer green and r2 primer blue are shown.
Are you sure you want to delete this list? Compared with other reverse transcription kits, this kit gives the highest dynamic range. The r1 primer is used for the measurement of total tRNA level, and the r2 primer is used for the measurement of ms 2 -modification level in individual tRNA. The first version of the LightMix H5 product has been tested on more than 50, samples in Asian countries and can be considered field-tested.
To compare the results between experiments, it is recommended to use same amount of RNA in all experiments. For uploading videos, you may need a Google account because Bio-protocol uses YouTube to host videos. The representative amplification curves using r1 primer red and r2 primer blue are shown. Thus, we successfully applied function tested RT—qPCR assays in our biomarker research for hypothesis generation with in vitro and in vivo models as well as for hypothesis testing with human FFPET samples.
It is recommended to use transcriptor first strand cdna synthesis kit roche pdf download method that can purify small RNAs [e. Cell Metab 21 3: Influenza virus A M2. For further processing only unless otherwise indicated. Deficiency of ms 2 modifications impairs mitochondrial protein synthesis, which transcriptor first strand cdna synthesis kit roche pdf download leads to the development of myopathy in mice and patients having mitochondrial diseases. Influenza A virus genome detection.
The mitochondrial tRNA is reversely transcribed by r1 primer and r2 primer, respectively.
Also remove everything in this list from your library. None Quantification Melting Curves None. Background – Selection of Sequences.
Fan-Yan Wei fywei kumamoto-u. The authors will be requested to answer your questions at their earliest convenience. Alternatively the detection of the features H5 and N1 transcriptkr of Influenza virus A can be ac- complished with the.
Data analysis Analyze data from Samples r1 and r2 to obtain Ct values. A generic error has occurred. Products — General Information.
The ms 2 modification in ms 2 i 6 A strengthens codon-anticodon interaction and contributes to accurate and efficient decoding.
LightMix Influenza a en
Set the program as follows and run real-time PCR. Because the dCt value precisely reflects the modification level Xie et al.
Measurement of ms 2 level of mt-tRNA Tyr in wild-type mouse heart tissue. Methods Volume 59, Issue 1JanuaryPages The product is lyophilized in 6 individual vials, 16 reactions each, providing a total of 96 reactions. It is recommended to prepare a master mixture of all reagents except cDNA to reduce variability.